Pall Fortebio Pioneer and Pioneer FE Systems

Pall Fortebio Pioneer and Pioneer FE Systems

The Pioneer platform offers high quality surface plasmon resonance (SPR) instruments for real-time, label-free measurements of biomolecular interactions.

Pioneer systems include measurement of molecules smaller than 70 daltons and provide insight into fragment analysis and protein aggregation. OneStep® Injections on the Pioneer platform use Taylor dispersion to generate an analyte concentration gradient that provides a high-resolution dose response in a single injection — allowing full analyte titration over 3-4 orders of magnitude in concentration within a few minutes. OneStep can also be used to determine the analyte diffusion coefficient and assess whether the analyte is heterogeneous or aggregated.

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Description

Key Features of Pioneer Systems for Binding Kinetics and Affinity

3 channel, fully automated SPR system
Eliminates time and error associated with making dilutions with OneStep gradient injections that acquire full kinetics titration in minutes automatically
Exquisite sensitivity and minimal noise for high affinity binding pairs
Up to 72 hours of unattended run time
Integrated, proven data analysis software based on Scrubber and Clamp platforms
Kretschmann optics for high sensitivity and stability

The Pioneer FE system enables SPR-based screening for identification of fragment candidates. Fragment screening is challenging with a large numbers of samples to process, low molecular weight analytes (<300 Da), and weak affinity interactions (KD: 10 µM to 10 mM). This combination of challenges makes it impractical to test binding above the KD and frequently results in making decisions based on small, square-shaped response curves. OneStep eliminates this by generating a continuous concentration gradient using the sample and running buffer. Weak affinity binding associated with fragments can be fitted with a real-time equilibrium isotherm model, since off-rates are fast enough to make the steady-state approximation. Competition assays are very useful in drug discovery, yielding the ability to find active site binders directly by competing fragment hits with a control molecule. Two sample components can be injected over the biosensor surface to perform a full kinetic analysis with site-specific competition using NeXtStep Injection technology.
Key Features of the Pioneer System for Fragment Screening

Make decisions early on with reliable affinity (KD) and kinetic (kd, ka) data directly from the primary screen
High throughput: up to 768 samples in 24 hours in a single experiment
The only SPR instrument to perform fragment binding competition assays in a single injection
Normalize screening data across different sensor chips, days, users or instruments to compare an entire screen campaign and select actives from the global data set

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