Complete solution for biomolecular interaction analysis with next generation SPR injections
Identify hits faster, obtain information-rich data and eliminate sample dilutions
With OneStep injections, users can obtain accurate kinetic and affinity values from one analyte concentration eliminating the need to run multiple analyte titrations saving time and error associated with making dilutions. NeXtStep injections provide a simple workflow to characterise competitive binders against a ligand to capture the mechanism of action.
Pioneer fluidic system is made up of inert materials such as PEEK, ceramic and Tefzal providing durability and low maintenance.
Extremely low baseline noise and drift enable measurement of reliable kinetics and affinity values for high affinity and challenging binders. With up to 40 Hz of data acquisition capabilities, Pioneer FE systems are capable of capturing very fast kinetic association and dissociation phases of a wide range of biomolecules and small molecule fragments.
3 channel, fully automated SPR system
With three sensing channels, two target proteins or ligands can be analysed simultaneously for binding with one surface utilised as a reference channel.
Screen 768 fragments in 24 hours
Pioneer FE platform’s high-throughput screening feature enables kinetic screening of 768 small molecule fragments in a single experiment in 24 hours.
Up to 72 hours of unattended run time
Offers a variety of sample vessel formats with up to two 384 well plate capacity with an unattended runtime of about 72 hrs for performing large binding screens.
Rapid data analysis with QDat software and hit selection feature
Pioneer SPR systems are equipped with an integrated, industry-proven data analysis software based on Scrubber and Clamp platforms. Raw data across multiple runs, days, and sensors can be combined and analysed using binding algorithms such as single site, multi-site binding, mass transport, and irreversible inhibitor analysis as required for analysis. Hit-selection software feature enables rapid identification of fragment hits that are acquired by Pioneer FE SPR systems.
Gain more data with less
As the analyte gradient is generated in OneStep injections, the diffusion coefficient is calculated that provides an assessment of aggregation enabling users to gain information not only on kinetics and affinity but also on aggregation in just one injection.
Pioneer systems accelerate the drug discovery process by providing fast, high quality kinetics to identify targets and characterise drug interactions. Perform high throughput fragment screening and characterise high affinity biologic interactions with confidence.
- Affinity & Kinetics
- Biologics & Small Molecule Research
- Fragment Based Drug Discovery
- Lead Characterisation
Characterisation of high affinity biologic interactions
Characterise irreversible inhibitors and measure commitment to covalency
High throughput fragment screening
Fragment-based drug design has become an increasingly popular platform for the identification of lead candidates in drug discovery programs. The detection and characterisation of fragment binding events is facilitated by sensitive biophysical technologies capable of detecting low affinity interactions of low molecular weight compounds. SPR has become one of the core technologies used in the identification of these low-affinity fragment compounds. SPR-based biosensors such as the Pioneer FE have the necessary sensitivity and throughput to provide complete fragment screens on libraries of several thousand compounds in just a few weeks per target.
Complete solution for biomolecular interaction analysis with Next Generation SPR Injections
Make decisions earlier in fragment screens with the Pioneer FE
|Number of channels; flowpaths||3 channels; 1, 1–2, 1–2–3, 3, 3–2, 3–2–1|
|Short term noise||< 0.1 RU||< 0.035 RU|
Limit of detection:
> 70 Da
102 – 108
10-6 – 0.1
> 70 Da
102 – 109
10-6 – 2.5
|Variable data rate||1 – 20 Hz||1 – 40 Hz|
|Sample temperature control||4 – 40 °C (max 15 °C below ambient)|
|Sample capacity and configuration||2 sample racks; 96 vial, deep well and PCR formats, 384 well microplates, custom high volume|
|Simultaneous injections; automation capabilities||Yes – dual sample loops; up to 72 hr unattended operation|
|High throughput screening mode||No||Yes|
|Qdat hit selection feature||No||Yes|
About Solve Scientific
Solve Scientific caters to the Australian and New Zealand scientific market with high-quality product and innovative technologies. In close partnership with our suppliers our role is delivering premium local support in enabling our customer’s results.