Benchtop system for measuring productivity by normalised titre
Combining titre with cell number which enables measures of specific productivity for both the static and expansion stages of cell line development
The award-winning new measure of productivity
From static stage to expansion and beyond, ICON™ is the world’s first benchtop system measuring normalised titre for the characterisation of high productivity clones in cell line development.
Winner of the SLAS New product Award, ICON offers low-volume measurements of critical quality attributes; IgG titre, confluency, cell number and viability cell density assessing productivity to enable rapid stratification of leading candidates.
From 2.5 to 1500 mg/L
Static plate cell count
For 1 – 24 samples
Brightfield and fluorescent image capture
Helping scientists focus on the winners, ICON enhances success through meaningful data
Viewing productivity by normalised titre
ICON combines titre with cell number enabling measures of specific productivity for both the static and expansion stages of cell line development
For cloning and expansion stages
- Rapid 96 well plate measurements
- Uses just 60 µl sample
- Wide detection range
Plate-based cell number
For cloning plate stage
- Rapid scanning
- High accuracy algorithm
- For 96 and 384 well plates
Automated viable cell counting
For expansion cell stage
- Trypan blue and AO/PI measurements
- From 1 to 24 counts per run
RAPID IgG QUANTIFICATION BY FLUORESCENCE POLARISATION
ICON Titre uses the fastest and easiest IgG assay available, fluorescence polarisation (FP). For use at either static or expansion stages, ICON Titre Plates offers a rapid plate-based assessment of IgG concentration. The plate is in 96-well format and is supplied pre-coated with an anti-FC fluorescent probe ready for use. The plate is designed for assessment on the ICON instrument using its fluorescence polarisation reader.
Each well requires 60 µL sample/pre-diluted sample. Two plate types are available. A ‘low’ range version measures a final concentration of 2.5 – 100 mg/L and a ‘PLUS’ range that measures 100 – 1500 mg/L.
FP is a well-documented naturally occurring phenomenon describing rotational diffusion, namely the observation that large complexes rotate slowly in solution and small complexes rotate more rapidly.
Plates are supplied pre-coated with an IgG-specific fluorescent probe. After a short incubation with supernatant or cell suspension, the plate is illuminated by polarised light. Where the probe binds to IgG, a large complex will form and relates to a reduction in emitted polarised light. This difference in rotation rate and thus in emitted light is compared against a standard curve, providing an accurate concentration quantification.
Viable cell density and automated cell counting
ICON offers rapid accurate automated cell counting and viability measurements for cells in suspension, enabling between 1 and 24 counts in a single run. The platform uses sterile disposable counting slides, each of which has eight cell counting positions. A slide rack enables three slides to be held in the instrument. Each sample requires just 20 µl of cell solution premixed with stain.
High clarity microscopy for cell line development
ICON Microscope Mode offers cell line development scientists a convenient set of tools for viewing and the capture of cellular images. Ideally suited for quick review and confirmation of cell morphology and fluorescent expression of RFP and GFP signals.
ICON is powered by STUDIUS
Smarter decision making in cell line development
Introducing the new standard for cell line development, STUDIUS™.
Integrated to work with Solentim’s technology platforms, STUDIUS manages and reports on cell line development data. Tracking each cell’s journey, STUDIUS offers dynamic data sorting to streamline the selection of candidate clones with desirable traits.
With Continuous Audit Mode, STUDIUS supports requirements to achieve 21 CFR part 11 and EU Annex 11 compliance.
- Manage: User management hierarchy including inbuilt user training course.
- HISTORYTREE™: Displays multiple data points from a clone’s history along a unique graphical timeline.
- Sort: Rapid filtering of per-cell data to characterize lead candidates.
- Report: Draws on system and service status within final report as evidence of quality process.
- Operates Continuous Audit Mode supporting requirements to achieve 21 CFR part 11 and EU Annex 11 compliance.
Follow the story with HISTORYTREE
Track every moment from seeding, evidence of clonality, growth, productivity through to selection.
Dynamic Sorting: hunting for those winning wells
Rapid dynamic sorting based on attributes including evidence of clonality, growth rate and productivity in the pursuit of lead candidates.
HISTORYTREE: a unique view of cell’s journey
HISTORYTREE is a graphical representation of a cell’s journey through cell line development. From seeding, evidence of clonality, growth, productivity and candidate selection, each node on the tree represents a time point in the cell’s journey offering easy access to critical data. Simple colour coding references data type.
Integrating with your cell line development ecosystem, STUDIUS imports valuable data from your VIPS™ and Cell Metric® instruments into one single management system.
Reporting: building in process and system quality
STUDIUS draws on system and service status, user accreditation and instrument performance within its final report as evidence of process quality.
Continuous Audit Mode
STUDIUS offers Continuous Audit Mode offering a range of data control related features are activated in line with clients’ ‘Part 11’ needs.
User Access with In-built Training
STUDIUS is supplied with a range of training tools to drive best practice and process quality control. These include online training, application and software user guides. Completion of video training course is connected to user accreditation and digital certification.
STUDIUS, controlling ICON
Easy control of the ICON instrument through Assay and Scan tabs. Clear management of sample source, scan set up and plate types. Vivid visual displays to guide user through streamlined workflow.
Benchtop system for measuring normalised titre in CLDCombining high efficiency seeding, in-well verification and high contrast whole well imaging to provide up-front assurance of clonality.
STUDIUS Data Management Platform
Smarter decision making in cell line developmentIntegrated to work with Solentim’s technology platforms, STUDIUS manages and reports on cell line development data.
About Solve Scientific
Solve Scientific caters to the Australian and New Zealand scientific market with high-quality product and innovative technologies. In close partnership with our suppliers our role is delivering premium local support in enabling our customer’s results.