SOLENTIM

VIPS AND VIPS PRO

VERIFIED IN-SITU PLATE SEEDING SYSTEMS

Solentim’s unrivalled All-in-One VIPS™ and VIPS PRO™ systems uniquely combine single cell seeding and whole well imaging, providing up-front assurance of clonality, saving time and money

TAKE SINGLE CELL CLONING TO A NEW LEVEL OF QUALITY AND CONFIDENCE

The Solentim VIPS™ and VIPS PRO™ systems take single cell cloning and image based clonality assurance to a whole new level of quality.  Theses multi-tasking, compact instruments deliver a rock solid, ‘double lock’ solution for regulatory submission.  With the VIPS seeders, two-round methodologies are a thing of the past.

Play Video

Elevating single cell seeding workflows

VIPS seeders accelerate projects.  With VIPS seeders you get healthier cells, more confirmed single cells per plate and better outgrowth.  High seeding efficiency for both 96 and 384-well plates, single round workflows for massively enhanced productivity and reduced timelines.

Delivering proof of monoclonality, not probability

With VIPS seeders, clonal assurance is based on evidence, unlike manual methods which use a repeated process to achieve probability of a single cell.  VIPS seeders deliver evidence at multiple steps for complete certainty of single cell seeding — not in the nozzle, not in the tube, not in the air, but at the bottom of the well.

Highest assurance of clonality 

Designed specifically for the task, Solentim technology is honed for single cell imaging in the droplet by way of z-stack imaging and in the whole well.  With high clarity images, even when near the cell wall, VIPS seeders generate confidence for the lab and the regulator.

DOWNLOAD BROCHURES FOR MORE INFORMATION

The Solentim ecosystem

Workflows for IND success

Assurance of clonality

Understanding the ‘double lock’ of clonal assurance

Single cell seeding with in-well verification

Operating at a pressure of less than one psi, VIPS seeders gently dispense cells in nanoliter droplets into a plate well.  Crucially, the cell is imaged in the bottom of the dry well as evidence of seeding.  Automated imaging in multiple tight z-stack layers of the whole droplet commences with intelligence-based image analysis confirming the existence of a single cell.  The well is then filled automatically with media.

Outgrowth and clonal characterisation

During the clonal growth phase, VIPS seeders perform daily high-clarity whole well imaging, recording a timeline of well images and performing analysis to measure confluency.  Successful clonal outgrowth can be traced back to a single cell to confirm monoclonality.

FEATURES

Designed to accelerate single-cell cloning workflows

Seeding for high clonal efficiency

For 96 and 384 well plates and quality single-project use consumables, we employ the simplest and safest method of single-cell dispensing, with no complex fluidics or mixed samples.   VIPS seeders use low pressure to seed cells in nanoliter droplets into empty wells.

In-line media dispensing

Immediately after confirmation of cell seeding, VIPS seeders automatically dispense culture media into the well, protecting your high-value cell.

Seeding for high clonal efficiency

For 96 and 384 well plates and quality single-project use consumables, we employ the simplest and safest method of single-cell dispensing, with no complex fluidics or mixed samples.   VIPS seeders use low pressure to seed cells in nanoliter droplets into empty wells.

In-line media dispensing

Immediately after confirmation of cell seeding, VIPS seeders automatically dispense culture media into the well, protecting your high-value cell.

In-well z-stack imaging

Immediately after seeding, the droplet is imaged at 20 different focal positions.  This broad z-stack encompasses the optimal plane for image analysis enabling confident single cell detection.

Always in focus ​

VIPS seeders perform total plate laser focus scanning, adjusting for plate contours, to ensure an optimal focal position for each well.

In-well z-stack imaging

Immediately after seeding, the droplet is imaged at 20 different focal positions.  This broad z-stack encompasses the optimal plane for image analysis enabling confident single cell detection.

Always in focus ​

VIPS seeders perform total plate laser focus scanning, adjusting for plate contours, to ensure an optimal focal position for each well.

Intelligent image analysis

Using advanced image analysis technology, z-stack images are processed to confirm the presence of a single cell.

QC verification process

To assist in the verification of your clonality workflow, the VIPS and VIPS PRO offer a verification methodology option based on rare-event fluorescence imaging.

Intelligent Image Analysis

Immediately after seeding, the droplet is imaged at 20 different focal positions.  This broad z-stack encompasses the optimal plane for image analysis enabling confident single cell detection.

QC verification process

To assist in the verification of your clonality workflow, the VIPS and VIPS PRO offer a verification methodology option based on rare-event fluorescence imaging.

SYSTEMS

VIPS System

High efficiency single cell cloning and whole well imaging

VIPS combines high efficiency seeding, in-well verification and high contrast whole well imaging to provide up-front assurance of clonality.

VIPS PRO System

Elevating single cell seeding for your GMP-certified workflows

 

VIPS PRO’s powerful seeding technology gently and efficiently dispenses cells, reducing hands-on time and compresses timelines from months to days. Now with solutions to support GMP-certification, VIPS PRO provides certainty of clonality through superior imaging, advanced data management and single-use GMP-compliant consumables.

VIPS PRO System

DOWNLOAD BROCHURES FOR MORE INFORMATION

The Solentim ecosystem

Workflows for IND success

Assurance of clonality

APPLICATIONS

Cell line development

The Solentim VIPS seeders accelerate single cell cloning workflows while also ensuring clonality to drive the discovery and development of antibody, cell & gene therapies and iPSC-based studies.

Single cell cloning
of hiPSCs

CLD for protein therapies

Virus manufacturing for gene therapy

High efficiency, high viability single cell cloning of hiPSCs for applications including development of disease models and supporting GMP manufacture of cell therapies

Generate stable cell lines that create producing novel recombinant proteins such as monoclonal antibodies or therapeutic proteins for rare diseases

From the selection of high producer clones to the development of stable vector producer cell lines, explore our simplified workflows for gene therapy applications

SINGLE CELL CLONING OF hiPSCs

High efficiency, high viability single cell cloning of hiPSCs for applications including development of disease models and supporting GMP manufacture of cell therapies

CLD FOR PROTEIN THERAPIES​

Generate stable cell lines that create producing novel recombinant proteins such as monoclonal antibodies or therapeutic proteins for rare diseases

VIRUS MANUFACTURING FOR GENE THERAPY​

From the selection of high producer clones to the development of stable vector producer cell lines, explore our simplified workflows for gene therapy applications

Vaccine production using mammalian cells

Engineered exosome producer cell lines

Cell engineering & precision gene editing

Isolate, screen and characterise attributes for clones producing novel recombinant sub-unit protein and monoclonal antibody vaccines

Establish stable producer cells for the production of engineered and cell-engineered exosomes

Optimise your drug discovery process using targeted gene editing to create stable cell lines as cellular reagents, for use in target validation, functional genomics and disease modelling

VACCINE PRODUCTION USING MAMMALIAN CELLS​

Isolate, screen and characterise attributes for clones producing novel recombinant sub-unit protein and monoclonal antibody vaccines

ENGINEERED EXOSOME PRODUCER CELL LINES​

Establish stable producer cells for the production of engineered and cell-engineered exosomes

CELL ENGINEERING & PRECISION GENE EDITING​

Optimise your drug discovery process using targeted gene editing to create stable cell lines as cellular reagents, for use in target validation, functional genomics and disease modelling

APPLICATION notes

Single cell seeding conditions for improved colony outgrowth

High efficiency single cell cloning of iPSCs

Clonality and improved outgrowth; developing cell lines for vector production

Scroll to Top