Gilson's trusted liquid handling tools for affinity purification workflows
Gilson offers automated, hands-free liquid handling solutions for performing affinity purification in biologics workflows. With various configurations and capacities available, Gilson has a solution to meet your needs, providing increased sample throughput and reliable test results.
Broad biologics applications
Whether it be for purification of antibody or HIS-tagged proteins, desalt cartridge prep or sample elution desalting, Gilson’s systems have the versatility to automate your biologic purification needs.
Parallel processing for maximum efficiency
Increase your efficiency and throughput on Gilson’s 274 systems, with up to 4 purifications performed in parallel and walk-away processing of samples.
Flexible configurations to suit your workflow
Gilson’s GX Liquid Handlers connect affinity columns inline for simple 1-step purifications. Gilson’s ASPECs have the additional utility to work with cartridges, allowing purification and desalt in the same process.
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Workflows for biomolecules
Guide to SPE automation
How it works
Whether purifying through affinity columns or cartridges, the workflow follow steps akin to common solid-phase extraction protocols, which can easily be applied to Gilson systems with commercially available consumables.
The versatility of Gilson’s line of automated liquid handlers (GX series) and solid phase extraction (ASPEC) systems enable a simple configuration transfer to perform affinity purification of biologics and antibodies. ASPEC Systems have increased utility, being capable of both column and cartridge formats for broader workflow versatility. Increase your efficiency and throughput on Gilson’s 274 systems, with up to 4 purifications performed in parallel and walk-away processing of samples.
GX liquid handler configurations
Single-step automated purification of mAbs on a Gilson GX liquid handler
For a single-step, small-scale purification with high purity results, monoclonal antibodies are purified using affinity columns. Conventional manual spin techniques or liquid chromatography systems are typical purification approaches, but it is also possible to purify antibodies with a simple liquid handler and syringe pump.
Configuring a Gilson liquid handling system for this workflow involves connecting the affinity column inline for buffer and sample aspiration through the column. During the elution step, the probes move to collection tubes on the bed to collect the eluted product off the column.
Scaled for low to medium throughput, a single-probe Gilson liquid handler can purify one antibody while a four-probe Gilson liquid handler can purify four antibodies simultaneously.
There are many considerations when moving from manual to automated mAb purification, and this technical note discusses some of the hardware and software considerations for maintaining sample integrity and reducing carryover when using a Gilson automated liquid handling system.
Automating mAb purification
ASPEC solid phase extraction configurations
Automated mid-scale parallel mAb purification on a Gilson ASPEC system
Protein purification is a bottleneck in protein production workflows. During a typical therapeutic antibody campaign, tens to hundreds of antibodies need to be purified from hybridoma cell supernatants for early characterisation. Gilson’s ASPEC Systems configured for mAb purification are simple to operate and fills a void in purification processes where a simple, efficient, automated system is needed for affinity purification of midsize research samples.
This paper describes the configuration of a 4-channel Gilson ASPEC System, set up perform 1-step and 2-step mAb affinity purifications using commercial affinity columns and desalt cartridges. By running 4 samples in parallel, this configuration has the capacity to purify up to 24 samples of greater than 50 mL each using a single-step affinity purification protocol, or a two-step protocol consisting of affinity chromatography followed by desalting/buffer exchange overnight (~12 h run time).
High-througput HIS-tagged protein purification
Automated high-throughput HIS-tagged protein purification on a Gilson ASPEC
The increasing need for high-throughput characterisation and production of pure proteins in drug discovery highlights the need for parallel and automated protein purification methods. The advantages of Gilson’s ASPEC configuration over existing methods is the combination of positive pressure extraction with high throughput, yield and reproducibility.
This paper describes the configuration of a 4-channel Gilson ASPEC System, set up perform 1-step HIS-tagged affinity purifications from cell lysates using commercial affinity resin packed in cartridges. By running 4 samples in parallel, this configuration allows fully automated purification of up to 60 cell lysates with milligram yields of pure recombinant protein in 18.5 h.
